B cell hyperresponsiveness and expansion of mature follicular B cells but not of marginal zone B cells in NFATc2/c3 double-deficient mice
Apstrakt
Marginal zone (MZ) B cells and peritoneal B-I cells provide a first defense system of thymus-independent Ab responses against foreign pathogens and therefore share a number of functional properties. Recently, development of B-1a cells was shown to be controlled by the transcription factor NFATc1. We show here that mice deficient for NFATc2 and c3 display a distinct lower representation of MZ B cells, which is correlated with a reduced capturing of trinitrophenyl-Ficoll. In contrast, mature follicular B cells from NFATc2/c3(-/-) mice are strongly increased in number. NFATc2/c3-/- B cells exhibit a marked increase in BCR-induced intracellular Ca(2+) mobilization and proliferation. However, trinitrophenyl-Ficoll-specific IgM and IgG3 responses of NFATc2/c3-deficient mice are intact, and chimeric mice reconstituted with NFATc2/3-deficient B cells show a normal number of MZ B cells and normal BCR responses. These observations suggest that the strongly elevated Th2 cytokine milieu in NFATc2/...c3-deficient mice leads to a hyperactivation of mature, follicular B cells, whereas MZ B cells are less responsive to these signals.
Izvor:
Journal of Immunology, 2005, 174, 8, 4797-4802Izdavač:
- Amer Assoc Immunologists, Bethesda
DOI: 10.4049/jimmunol.174.8.4797
ISSN: 0022-1767
PubMed: 15814705
WoS: 000228234600045
Scopus: 2-s2.0-17044439536
Institucija/grupa
rFASPERTY - JOUR AU - Samanta, DN AU - Palmetshofer, A AU - Marinković, Dragan AU - Wirth, Thomas AU - Serfling, E AU - Nitschke, L. PY - 2005 UR - http://rfasper.fasper.bg.ac.rs/handle/123456789/37 AB - Marginal zone (MZ) B cells and peritoneal B-I cells provide a first defense system of thymus-independent Ab responses against foreign pathogens and therefore share a number of functional properties. Recently, development of B-1a cells was shown to be controlled by the transcription factor NFATc1. We show here that mice deficient for NFATc2 and c3 display a distinct lower representation of MZ B cells, which is correlated with a reduced capturing of trinitrophenyl-Ficoll. In contrast, mature follicular B cells from NFATc2/c3(-/-) mice are strongly increased in number. NFATc2/c3-/- B cells exhibit a marked increase in BCR-induced intracellular Ca(2+) mobilization and proliferation. However, trinitrophenyl-Ficoll-specific IgM and IgG3 responses of NFATc2/c3-deficient mice are intact, and chimeric mice reconstituted with NFATc2/3-deficient B cells show a normal number of MZ B cells and normal BCR responses. These observations suggest that the strongly elevated Th2 cytokine milieu in NFATc2/c3-deficient mice leads to a hyperactivation of mature, follicular B cells, whereas MZ B cells are less responsive to these signals. PB - Amer Assoc Immunologists, Bethesda T2 - Journal of Immunology T1 - B cell hyperresponsiveness and expansion of mature follicular B cells but not of marginal zone B cells in NFATc2/c3 double-deficient mice EP - 4802 IS - 8 SP - 4797 VL - 174 DO - 10.4049/jimmunol.174.8.4797 ER -
@article{ author = "Samanta, DN and Palmetshofer, A and Marinković, Dragan and Wirth, Thomas and Serfling, E and Nitschke, L.", year = "2005", abstract = "Marginal zone (MZ) B cells and peritoneal B-I cells provide a first defense system of thymus-independent Ab responses against foreign pathogens and therefore share a number of functional properties. Recently, development of B-1a cells was shown to be controlled by the transcription factor NFATc1. We show here that mice deficient for NFATc2 and c3 display a distinct lower representation of MZ B cells, which is correlated with a reduced capturing of trinitrophenyl-Ficoll. In contrast, mature follicular B cells from NFATc2/c3(-/-) mice are strongly increased in number. NFATc2/c3-/- B cells exhibit a marked increase in BCR-induced intracellular Ca(2+) mobilization and proliferation. However, trinitrophenyl-Ficoll-specific IgM and IgG3 responses of NFATc2/c3-deficient mice are intact, and chimeric mice reconstituted with NFATc2/3-deficient B cells show a normal number of MZ B cells and normal BCR responses. These observations suggest that the strongly elevated Th2 cytokine milieu in NFATc2/c3-deficient mice leads to a hyperactivation of mature, follicular B cells, whereas MZ B cells are less responsive to these signals.", publisher = "Amer Assoc Immunologists, Bethesda", journal = "Journal of Immunology", title = "B cell hyperresponsiveness and expansion of mature follicular B cells but not of marginal zone B cells in NFATc2/c3 double-deficient mice", pages = "4802-4797", number = "8", volume = "174", doi = "10.4049/jimmunol.174.8.4797" }
Samanta, D., Palmetshofer, A., Marinković, D., Wirth, T., Serfling, E.,& Nitschke, L.. (2005). B cell hyperresponsiveness and expansion of mature follicular B cells but not of marginal zone B cells in NFATc2/c3 double-deficient mice. in Journal of Immunology Amer Assoc Immunologists, Bethesda., 174(8), 4797-4802. https://doi.org/10.4049/jimmunol.174.8.4797
Samanta D, Palmetshofer A, Marinković D, Wirth T, Serfling E, Nitschke L. B cell hyperresponsiveness and expansion of mature follicular B cells but not of marginal zone B cells in NFATc2/c3 double-deficient mice. in Journal of Immunology. 2005;174(8):4797-4802. doi:10.4049/jimmunol.174.8.4797 .
Samanta, DN, Palmetshofer, A, Marinković, Dragan, Wirth, Thomas, Serfling, E, Nitschke, L., "B cell hyperresponsiveness and expansion of mature follicular B cells but not of marginal zone B cells in NFATc2/c3 double-deficient mice" in Journal of Immunology, 174, no. 8 (2005):4797-4802, https://doi.org/10.4049/jimmunol.174.8.4797 . .