B cell-specific transgenic expression of Bcl2 rescues early B lymphopoiesis but not B cell responses in BOB.1/OBF.1-deficient mice
Апстракт
Mice deficient for the transcriptional coactivator BOB.1/OBF.1 show several defects in B cell differentiation. Numbers of immature transitional B cells in the bone marrow are reduced and fewer B cells reach the periphery. Furthermore, germinal center B cells are absent and marginal zone (MZ) B lymphocytes are markedly reduced. Increased levels of B cell apoptosis in these mice prompted us to analyze expression and function of antiapoptotic proteins. Bcl2 expression is strongly reduced in BOB.1/OBF.1-deficient pre-B cells. When BOB.1/OBF.1-deficient mice were crossed with Bcl2-transgenic mice, B cell development in the bone marrow and numbers of B cells in peripheral lymphoid organs were normalized. However, neither germinal center B cells nor MZ B cells were rescued. Additionally, Bcl2 did not rescue the defects in signaling and affinity maturation found in BOB.1/OBF.1-deficient mice. Interestingly, Bcl2-transgenic mice by themselves show an MZ B cell defect. Virtually no functional MZ... B cells were detected in these mice. In contrast, mice deficient for Bcl2 show a relative increase in MZ B cell numbers, indicating a previously undetected function of Bcl2 for this B cell compartment.
Кључне речи:
B cell differentiation / germinal center reaction / affinity maturation / marginal zone / transcription factorИзвор:
Journal of Experimental Medicine, 2003, 197, 9, 1205-1211Издавач:
- Rockefeller Univ Press, New York
DOI: 10.1084/jem.20022014
ISSN: 0022-1007
PubMed: 12732662
WoS: 000182752000015
Scopus: 2-s2.0-0038219633
Институција/група
rFASPERTY - JOUR AU - Brunner, C AU - Marinković, Dragan AU - Klein, J AU - Samardžić, T. AU - Nitschke, L. AU - Wirth, Thomas PY - 2003 UR - http://rfasper.fasper.bg.ac.rs/handle/123456789/31 AB - Mice deficient for the transcriptional coactivator BOB.1/OBF.1 show several defects in B cell differentiation. Numbers of immature transitional B cells in the bone marrow are reduced and fewer B cells reach the periphery. Furthermore, germinal center B cells are absent and marginal zone (MZ) B lymphocytes are markedly reduced. Increased levels of B cell apoptosis in these mice prompted us to analyze expression and function of antiapoptotic proteins. Bcl2 expression is strongly reduced in BOB.1/OBF.1-deficient pre-B cells. When BOB.1/OBF.1-deficient mice were crossed with Bcl2-transgenic mice, B cell development in the bone marrow and numbers of B cells in peripheral lymphoid organs were normalized. However, neither germinal center B cells nor MZ B cells were rescued. Additionally, Bcl2 did not rescue the defects in signaling and affinity maturation found in BOB.1/OBF.1-deficient mice. Interestingly, Bcl2-transgenic mice by themselves show an MZ B cell defect. Virtually no functional MZ B cells were detected in these mice. In contrast, mice deficient for Bcl2 show a relative increase in MZ B cell numbers, indicating a previously undetected function of Bcl2 for this B cell compartment. PB - Rockefeller Univ Press, New York T2 - Journal of Experimental Medicine T1 - B cell-specific transgenic expression of Bcl2 rescues early B lymphopoiesis but not B cell responses in BOB.1/OBF.1-deficient mice EP - 1211 IS - 9 SP - 1205 VL - 197 DO - 10.1084/jem.20022014 ER -
@article{ author = "Brunner, C and Marinković, Dragan and Klein, J and Samardžić, T. and Nitschke, L. and Wirth, Thomas", year = "2003", abstract = "Mice deficient for the transcriptional coactivator BOB.1/OBF.1 show several defects in B cell differentiation. Numbers of immature transitional B cells in the bone marrow are reduced and fewer B cells reach the periphery. Furthermore, germinal center B cells are absent and marginal zone (MZ) B lymphocytes are markedly reduced. Increased levels of B cell apoptosis in these mice prompted us to analyze expression and function of antiapoptotic proteins. Bcl2 expression is strongly reduced in BOB.1/OBF.1-deficient pre-B cells. When BOB.1/OBF.1-deficient mice were crossed with Bcl2-transgenic mice, B cell development in the bone marrow and numbers of B cells in peripheral lymphoid organs were normalized. However, neither germinal center B cells nor MZ B cells were rescued. Additionally, Bcl2 did not rescue the defects in signaling and affinity maturation found in BOB.1/OBF.1-deficient mice. Interestingly, Bcl2-transgenic mice by themselves show an MZ B cell defect. Virtually no functional MZ B cells were detected in these mice. In contrast, mice deficient for Bcl2 show a relative increase in MZ B cell numbers, indicating a previously undetected function of Bcl2 for this B cell compartment.", publisher = "Rockefeller Univ Press, New York", journal = "Journal of Experimental Medicine", title = "B cell-specific transgenic expression of Bcl2 rescues early B lymphopoiesis but not B cell responses in BOB.1/OBF.1-deficient mice", pages = "1211-1205", number = "9", volume = "197", doi = "10.1084/jem.20022014" }
Brunner, C., Marinković, D., Klein, J., Samardžić, T., Nitschke, L.,& Wirth, T.. (2003). B cell-specific transgenic expression of Bcl2 rescues early B lymphopoiesis but not B cell responses in BOB.1/OBF.1-deficient mice. in Journal of Experimental Medicine Rockefeller Univ Press, New York., 197(9), 1205-1211. https://doi.org/10.1084/jem.20022014
Brunner C, Marinković D, Klein J, Samardžić T, Nitschke L, Wirth T. B cell-specific transgenic expression of Bcl2 rescues early B lymphopoiesis but not B cell responses in BOB.1/OBF.1-deficient mice. in Journal of Experimental Medicine. 2003;197(9):1205-1211. doi:10.1084/jem.20022014 .
Brunner, C, Marinković, Dragan, Klein, J, Samardžić, T., Nitschke, L., Wirth, Thomas, "B cell-specific transgenic expression of Bcl2 rescues early B lymphopoiesis but not B cell responses in BOB.1/OBF.1-deficient mice" in Journal of Experimental Medicine, 197, no. 9 (2003):1205-1211, https://doi.org/10.1084/jem.20022014 . .