TY  - JOUR
AU  - Kokai, Enikoe
AU  - Voss, Florian
AU  - Fleischer, Frank
AU  - Kempe, Sybille
AU  - Marinković, Dragan
AU  - Wolburg, Hartwig
AU  - Leithaeuser, Frank
AU  - Schmidt, Volker
AU  - Deutsch, Urban
AU  - Wirth, Thomas
PY  - 2009
UR  - http://rfasper.fasper.bg.ac.rs/handle/123456789/252
AB  - Previous work has shown that c-Myc is required for adequate vasculogenesis and angiogenesis. To further investigate the contribution of Myc to these processes, we conditionally expressed c-Myc in embryonic endothelial cells using a tetracycline-regulated system. Endothelial Myc overexpression resulted in severe defects in the embryonic vascular system. Myc-expressing embryos undergo widespread edema formation and multiple hemorrhagic lesions. They die between embryonic days 14.5 and 17.5. The changes in vascular permeability are not caused by deficiencies in vascular basement membrane composition or pericyte coverage. However, the overall turnover of endothelial cells is elevated as is revealed by increased levels of both proliferation and apoptosis. Whole-mount immunohistochemical analysis revealed alterations in the architecture of capillary networks. The dermal vasculature of Myc-expressing embryos is characterized by a reduction in vessel branching, which occurs despite upregulation of the proangiogenic factors vascular endothelial growth factor-A and angiopoietin-2. Thus, the net outcome of an excess of vascular endothelial growth factor-A and angiopoietin-2 in the face of an elevated cellular turnover appears to be a defect in vascular integrity. (Circ Res. 2009;104:1151-1159.)
PB  - Lippincott Williams & Wilkins, Philadelphia
T2  - Circulation Research
T1  - Myc Regulates Embryonic Vascular Permeability and Remodeling
EP  - U59
IS  - 10
SP  - 1151
VL  - 104
DO  - 10.1161/CIRCRESAHA.108.191460
ER  - 

@article{
author = "Kokai, Enikoe and Voss, Florian and Fleischer, Frank and Kempe, Sybille and Marinković, Dragan and Wolburg, Hartwig and Leithaeuser, Frank and Schmidt, Volker and Deutsch, Urban and Wirth, Thomas",
year = "2009",
abstract = "Previous work has shown that c-Myc is required for adequate vasculogenesis and angiogenesis. To further investigate the contribution of Myc to these processes, we conditionally expressed c-Myc in embryonic endothelial cells using a tetracycline-regulated system. Endothelial Myc overexpression resulted in severe defects in the embryonic vascular system. Myc-expressing embryos undergo widespread edema formation and multiple hemorrhagic lesions. They die between embryonic days 14.5 and 17.5. The changes in vascular permeability are not caused by deficiencies in vascular basement membrane composition or pericyte coverage. However, the overall turnover of endothelial cells is elevated as is revealed by increased levels of both proliferation and apoptosis. Whole-mount immunohistochemical analysis revealed alterations in the architecture of capillary networks. The dermal vasculature of Myc-expressing embryos is characterized by a reduction in vessel branching, which occurs despite upregulation of the proangiogenic factors vascular endothelial growth factor-A and angiopoietin-2. Thus, the net outcome of an excess of vascular endothelial growth factor-A and angiopoietin-2 in the face of an elevated cellular turnover appears to be a defect in vascular integrity. (Circ Res. 2009;104:1151-1159.)",
publisher = "Lippincott Williams & Wilkins, Philadelphia",
journal = "Circulation Research",
title = "Myc Regulates Embryonic Vascular Permeability and Remodeling",
pages = "U59-1151",
number = "10",
volume = "104",
doi = "10.1161/CIRCRESAHA.108.191460"
}

Kokai, E., Voss, F., Fleischer, F., Kempe, S., Marinković, D., Wolburg, H., Leithaeuser, F., Schmidt, V., Deutsch, U.,& Wirth, T.. (2009). Myc Regulates Embryonic Vascular Permeability and Remodeling. in Circulation Research
Lippincott Williams & Wilkins, Philadelphia., 104(10), 1151-U59.
https://doi.org/10.1161/CIRCRESAHA.108.191460

Kokai E, Voss F, Fleischer F, Kempe S, Marinković D, Wolburg H, Leithaeuser F, Schmidt V, Deutsch U, Wirth T. Myc Regulates Embryonic Vascular Permeability and Remodeling. in Circulation Research. 2009;104(10):1151-U59.
doi:10.1161/CIRCRESAHA.108.191460 .

Kokai, Enikoe, Voss, Florian, Fleischer, Frank, Kempe, Sybille, Marinković, Dragan, Wolburg, Hartwig, Leithaeuser, Frank, Schmidt, Volker, Deutsch, Urban, Wirth, Thomas, "Myc Regulates Embryonic Vascular Permeability and Remodeling" in Circulation Research, 104, no. 10 (2009):1151-U59,
https://doi.org/10.1161/CIRCRESAHA.108.191460 . .

TY  - JOUR
AU  - Marinković, Dragan
AU  - Marinković, Tatjana
AU  - Kokai, Enikoe
AU  - Barth, T.
AU  - Moller, P.
AU  - Wirth, Thomas
PY  - 2004
UR  - http://rfasper.fasper.bg.ac.rs/handle/123456789/33
AB  - The c-Myc transcription factor regulates a wide set of genes involved in processes such as proliferation, differentiation and apoptosis. Therefore, altered expression of Myc leads to deregulation of a large number of target genes and, as a consequence, to tumorigenesis. For understanding Myc-induced transformation, identification of these target genes is essential. In this study, we searched for Myc target genes involved in lymphomagenesis using different mouse T and B cell lymphoma cell lines transformed by a conditional Myc-allele. Target genes obtained by microarray experiments were further subjected to a kinetic analysis of mRNA expression upon Myc inactivation/reactivation, bioinformatic examination of Myc binding sites and chromatin immunoprecipitation. This approach allowed us to define targets whose activation is a direct consequence of Myc binding. Among the 38 novel Myc targets, we identified several genes implicated in the tumor development. These genes are not only relevant for mouse lymphomas because we observed their upregulation in human lymphomas as well. Our findings further the understanding of Myc-induced lymphomagenesis and help toward developing more efficient antitumor strategies.
PB  - Oxford Univ Press, Oxford
T2  - Nucleic Acids Research
T1  - Identification of novel Myc target genes with a potential role in lymphomagenesis
EP  - 5378
IS  - 18
SP  - 5368
VL  - 32
DO  - 10.1093/nar/gkh877
ER  - 

@article{
author = "Marinković, Dragan and Marinković, Tatjana and Kokai, Enikoe and Barth, T. and Moller, P. and Wirth, Thomas",
year = "2004",
abstract = "The c-Myc transcription factor regulates a wide set of genes involved in processes such as proliferation, differentiation and apoptosis. Therefore, altered expression of Myc leads to deregulation of a large number of target genes and, as a consequence, to tumorigenesis. For understanding Myc-induced transformation, identification of these target genes is essential. In this study, we searched for Myc target genes involved in lymphomagenesis using different mouse T and B cell lymphoma cell lines transformed by a conditional Myc-allele. Target genes obtained by microarray experiments were further subjected to a kinetic analysis of mRNA expression upon Myc inactivation/reactivation, bioinformatic examination of Myc binding sites and chromatin immunoprecipitation. This approach allowed us to define targets whose activation is a direct consequence of Myc binding. Among the 38 novel Myc targets, we identified several genes implicated in the tumor development. These genes are not only relevant for mouse lymphomas because we observed their upregulation in human lymphomas as well. Our findings further the understanding of Myc-induced lymphomagenesis and help toward developing more efficient antitumor strategies.",
publisher = "Oxford Univ Press, Oxford",
journal = "Nucleic Acids Research",
title = "Identification of novel Myc target genes with a potential role in lymphomagenesis",
pages = "5378-5368",
number = "18",
volume = "32",
doi = "10.1093/nar/gkh877"
}

Marinković, D., Marinković, T., Kokai, E., Barth, T., Moller, P.,& Wirth, T.. (2004). Identification of novel Myc target genes with a potential role in lymphomagenesis. in Nucleic Acids Research
Oxford Univ Press, Oxford., 32(18), 5368-5378.
https://doi.org/10.1093/nar/gkh877

Marinković D, Marinković T, Kokai E, Barth T, Moller P, Wirth T. Identification of novel Myc target genes with a potential role in lymphomagenesis. in Nucleic Acids Research. 2004;32(18):5368-5378.
doi:10.1093/nar/gkh877 .

Marinković, Dragan, Marinković, Tatjana, Kokai, Enikoe, Barth, T., Moller, P., Wirth, Thomas, "Identification of novel Myc target genes with a potential role in lymphomagenesis" in Nucleic Acids Research, 32, no. 18 (2004):5368-5378,
https://doi.org/10.1093/nar/gkh877 . .